Saponin Biosynthesis in Saponaria vaccaria. cDNAs Encoding β-Amyrin Synthase and a Triterpene Carboxylic Acid Glucosyltransferase
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Saponin Biosynthesis in Saponaria vaccaria. cDNAs Encoding b-Amyrin Synthase and a Triterpene Carboxylic Acid Glucosyltransferase
Saponaria vaccaria (Caryophyllaceae), a soapwort, known in western Canada as cowcockle, contains bioactive oleanane-type saponins similar to those found in soapbark tree (Quillaja saponaria; Rosaceae). To improve our understanding of the biosynthesis of these saponins, a combined polymerase chain reaction and expressed sequence tag approach was taken to identify the genes involved. A cDNA encod...
متن کاملSaponin biosynthesis in Saponaria vaccaria. cDNAs encoding beta-amyrin synthase and a triterpene carboxylic acid glucosyltransferase.
Saponaria vaccaria (Caryophyllaceae), a soapwort, known in western Canada as cowcockle, contains bioactive oleanane-type saponins similar to those found in soapbark tree (Quillaja saponaria; Rosaceae). To improve our understanding of the biosynthesis of these saponins, a combined polymerase chain reaction and expressed sequence tag approach was taken to identify the genes involved. A cDNA encod...
متن کاملMolecular Biology of Triterpenoid Biosynthesis in Glycyrrhiza Plants
Licorice (roots and stolons of Glycyrrhiza plants, Leguminosae) is a well-known natural sweetener, and its sweet constituent is an oleanane-type triterpene saponin, glycyrrhizin. Cultured cells of G. glabra do not produce glycyrrhizin, but produce two structurally different triterpenoid constituents, betulinic acid and soyasaponins. Both glycyrrhizin and soyasaponins are oleanane-type triterpen...
متن کاملβ‐Amyrin synthase from Conyza blinii expressed in Saccharomyces cerevisiae
Conyza blinii H.Lév. is a widely used medicinal herb in southwestern China. The main pharmacological components of C. blinii are a class of oleanane-type pentacyclic triterpene glycosides known as conyzasaponins, which are thought to be synthesized from β-amyrin. However, no genes involved in the conyzasaponin pathway have previously been identified. Here, we identify an oxidosqualene cyclase (...
متن کاملTriterpene functional genomics in licorice for identification of CYP72A154 involved in the biosynthesis of glycyrrhizin.
Glycyrrhizin, a triterpenoid saponin derived from the underground parts of Glycyrrhiza plants (licorice), has several pharmacological activities and is also used worldwide as a natural sweetener. The biosynthesis of glycyrrhizin involves the initial cyclization of 2,3-oxidosqualene to the triterpene skeleton β-amyrin, followed by a series of oxidative reactions at positions C-11 and C-30, and g...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 2006
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.106.088484